NCOA1 anticorps (pTyr416)

N° du produit ABIN126893

L’anticorps Souris Monoclonal anti-NCOA1 a été validé pour WB, EIA, IF et FACS. Il convient pour détecter NCOA1 dans des échantillons de Humain et Souris.

Aperçu rapide pour NCOA1 anticorps (pTyr416) (ABIN126893)

Antigène: NCOA1 (Nuclear Receptor Coactivator 1 (NCOA1))

Reactivité: Humain, Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp NCOA1 est non-conjugé

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: 9A6

Détail du produit anti-NCOA1 anticorps

Épitope: pTyr416

Specificité: AM00145PU-N specifically interacts with activated src phosphorylated at Tyr 416 and with src family kinases phosphorylated at the corresponding Tyr in the activation loop. The antibody does not crossreact with the non-phosphorylated form of src nor with unrelated phosphorylation sites.

Attributs du produit: Synonyms: c-Src, pp60c-src, Proto-oncogene tyrosine-protein kinase Src, Proto-oncogene c-Src

Purification: Subsequent Thiophilic Adsorption and Size Exclusion Chromatography.

Immunogène: Synthetic phosphopeptide conjuagted to KLHEpitope: phosphotyrosine 416AA Sequence: DNEpYTAR

Isotype: IgG3

Information d'application

Indications d'application: Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. ELISA: 0.05 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore with 1.0 mL H2O (15 min, RT).

Buffer: 2 x PBS / 0.09 % Sodium Azide / PEG and Sucrose.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: For long-term storage, freeze lyophilizate upon arrival (-20 °C). Upon reconstitution, aliquote and freeze in liquid nitrogen, reconstituted antibody can bestored frozen at -80 °C up to 1 year. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months. Avoid repeated freezing and thawing.

Date de péremption: 12 months

Détails sur NCOA1

Antigène: NCOA1 (Nuclear Receptor Coactivator 1 (NCOA1))

Autre désignation: SRC1

Sujet: Src is the best understood member of a family of 9 tyrosine kinases regulating cellular responses to extracellular stimuli. Src is activated by PDGF and other growth factors that activate transmembrane tyrosine kinase receptors as well as increased intracellular calcium or oxidative stress. Src becomes activated upon dephosphorylation of Tyr 527, leading to a conformational change of the src molecule towards an "open", active conformation which allows binding to other proteins via the SH2 and SH3 domain. Activated src phosphorylates a variety of substrates involved in the mitogenic signalling pathway, cytoskeletal organization, cell-substrate adhesion, cell-cell adhesion, cell-cell communication, and RNA processing.Synonyms: Proto-oncogene c-Src, Proto-oncogene tyrosine-protein kinase Src, c-Src, pp60c-src

ID gène: 6714

UniProt: P12931

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Chromatin Binding, Regulation of Lipid Metabolism by PPARalpha